vizgen merscope (Vizgen Inc)
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Vizgen Merscope, supplied by Vizgen Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/merscope+system/merscope+vizgen+%E2%80%A2/pm42162410-27-20-20
Average 86 stars, based on 1 article reviews
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other:Article Title: Single-cell transcriptomic and genomic changes in the ageing human brain. Article Snippet: Article Title: Brain-wide connectivity and novelty response of the dorsal endopiriform nucleus in mice Article Snippet: Once the hydrogel solidified (~1.5 h), the samples were cleared overnight at 37°C with a Clearing Solution containing Proteinase K (NEB P8107S) and Article Title: Single-cell spatial transcriptomic profiling defines a pathogenic inflammatory niche in chronic active multiple sclerosis lesions. Article Snippet: Immunohistochemistry and sample punching of human brain tissue For immunohistochemistry, human brain sections were stained with primary antibodies against MOG (Abcam, catalogue no. ab109746, 1:125), MHC-II (Dako, clone CR3/43, catalogue no. M0775, 1:25), BCAS1 (Santa Cruz Biotechnology, catalogue no. sc-136342, 1:250), and CD3 (DAKO, catalogue no. A0452, 1:50) according to previously published protocols.56,57 Antibody binding was visualized using biotinylated secondary antibodies, peroxidase-conjugated avidin, DAB (Sigma-Aldrich) and Fast Blue using an alkaline phosphatase conjugated secondary antibody (Dako, 1:50). Imaging:Article Title: Aberrant host mRNA partitioning in Ebola virus condensates driven by RNA folding perpetuates species-dependent interferon response Article Snippet: After removing clearing solution and washing three times with Sample Prep Wash Buffer (Vizgen 20300001), the samples were stained with DAPI and Poly T Reagent (Vizgen 20300021) for 15 minutes at room temperature, washed for 10 minutes with 5 ml of Formamide Wash Buffer (Vizgen 20300002), then washed for 5 minutes with 5 ml of Sample Prep Wash Buffer (Vizgen 20300001). .. The imaging reagents and processed sample were loaded into the MERSCOPE and a low-resolution DAPI mosaic (10X magnification) was used to select the region of interest before high-resolution imaging on the Article Title: Molecular and spatial transcriptomic classification of midbrain dopamine neurons and their alterations in a LRRK2 G2019S model of Parkinson’s disease Article Snippet: .. Once processed, brain sections were prepped for imaging using the commercial Article Title: TREM2 macrophages are associated with enhanced response to PD-1 blockade in human hepatocellular carcinoma Article Snippet: Imaging buffer was prepared by adding Imaging Buffer Activator (Vizgen 20300015) and RNase inhibitor to the Imaging Buffer Base. .. Processed samples and imaging reagents were loaded into the Article Title: Microglia activation orchestrates CXCL10-mediated CD8 + T cell recruitment to promote aging-related white matter degeneration. Article Snippet: .. The samples were then washed, and the appropriate hybridization and imaging buffers were loaded onto the Fluorescence:Article Title: SAME: Topology-flexible transforms enable robust integration of multimodal spatial omics Article Snippet: .. Multiplexed error-robust fluorescence in situ hybridization (MERFISH) was carried out using the In Situ Hybridization:Article Title: SAME: Topology-flexible transforms enable robust integration of multimodal spatial omics Article Snippet: .. Multiplexed error-robust fluorescence in situ hybridization (MERFISH) was carried out using the Hybridization:Article Title: Microglia activation orchestrates CXCL10-mediated CD8 + T cell recruitment to promote aging-related white matter degeneration. Article Snippet: .. The samples were then washed, and the appropriate hybridization and imaging buffers were loaded onto the |
